6FFH

Crystal Structure of mGluR5 in complex with Fenobam at 2.65 A

6FFH の概要

• エントリーDOI: 10.2210/pdb6ffh/pdb
• 分子名称: Metabotropic glutamate receptor 5,Endolysin, OLEIC ACID, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (6 entities in total)
• 機能のキーワード: 7tm, receptor, gpcr, membrane-protein, signaling protein, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 52090.35

構造登録者

Christopher, J.A.,Orgovan, Z.,Congreve, M.,Dore, A.S.,Errey, J.C.,Marshall, F.H.,Mason, J.S.,Okrasa, K.,Rucktooa, P.,Serrano-Vega, M.J.,Ferenczy, G.G.,Keseru, G.M. (登録日: 2018-01-08, 公開日: 2018-03-07, 最終更新日: 2024-01-17)

主引用文献

Christopher, J.A.,Orgovan, Z.,Congreve, M.,Dore, A.S.,Errey, J.C.,Marshall, F.H.,Mason, J.S.,Okrasa, K.,Rucktooa, P.,Serrano-Vega, M.J.,Ferenczy, G.G.,Keseru, G.M.
Structure-Based Optimization Strategies for G Protein-Coupled Receptor (GPCR) Allosteric Modulators: A Case Study from Analyses of New Metabotropic Glutamate Receptor 5 (mGlu5) X-ray Structures.
J.Med.Chem., 62:207-222, 2019

PubMed Abstract: Two interesting new X-ray structures of negative allosteric modulator (NAM) ligands for the mGlu receptor, M-MPEP (3) and fenobam (4), are reported. The new structures show how the binding of the ligands induces different receptor water channel conformations to previously published structures. The structure of fenobam, where a urea replaces the acetylenic linker in M-MPEP and mavoglurant, reveals a binding mode where the ligand is rotated by 180° compared to a previously proposed docking model. The need for multiple ligand structures for accurate GPCR structure-based drug design is demonstrated by the different growing vectors identified for the head groups of M-MPEP and mavoglurant and by the unexpected water-mediated receptor interactions of a new chemotype represented by fenobam. The implications of the new structures for ligand design are discussed, with extensive analysis of the energetics of the water networks of both pseudoapo and bound structures providing a new design strategy for allosteric modulators.

PubMed: 29455526
DOI: 10.1021/acs.jmedchem.7b01722

実験手法

X-RAY DIFFRACTION (2.65 Å)