6FE8

Cryo-EM structure of the core Centromere Binding Factor 3 complex

6FE8 の概要

• エントリーDOI: 10.2210/pdb6fe8/pdb
• 関連するPDBエントリー: 6GSA
• EMDBエントリー: 0051 4241
• 分子名称: Centromere DNA-binding protein complex CBF3 subunit B, Suppressor of kinetochore protein 1, Centromere DNA-binding protein complex CBF3 subunit C (3 entities in total)
• 機能のキーワード: centromere, cdeiii-binding, lrr domain, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 220366.66

構造登録者

Zhang, W.J.,Lukoynova, N.,Miah, S.,Vaughan, C.K. (登録日: 2017-12-30, 公開日: 2018-08-01, 最終更新日: 2024-11-20)

主引用文献

Zhang, W.,Lukoynova, N.,Miah, S.,Lucas, J.,Vaughan, C.K.
Insights into Centromere DNA Bending Revealed by the Cryo-EM Structure of the Core Centromere Binding Factor 3 with Ndc10.
Cell Rep, 24:744-754, 2018

PubMed Abstract: The centromere binding factor 3 (CBF3) complex binds the third centromere DNA element in organisms with point centromeres, such as S. cerevisiae. It is an essential complex for assembly of the kinetochore in these organisms, as it facilitates genetic centromere specification and allows association of all other kinetochore components. We determined high-resolution structures of the core complex of CBF3 alone and in association with a monomeric construct of Ndc10, using cryoelectron microscopy (cryo-EM). We identify the DNA-binding site of the complex and present a model in which CBF3 induces a tight bend in centromeric DNA, thus facilitating assembly of the centromeric nucleosome.

PubMed: 30021170
DOI: 10.1016/j.celrep.2018.06.068

実験手法

ELECTRON MICROSCOPY (4.1 Å)