6FC7

Crystal Structure of Two-Domain Laccase mutant H165F from Streptomyces griseoflavus with high copper ions occupancy

6FC7 の概要

• エントリーDOI: 10.2210/pdb6fc7/pdb
• 関連するPDBエントリー: 5MKM
• 分子名称: Two-domain laccase, COPPER (II) ION, PEROXIDE ION, ... (6 entities in total)
• 機能のキーワード: two-domain laccase, laccase, streptomyces griseoflavus, oxidoreductase
• 由来する生物種: Streptomyces griseoflavus
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 420526.26

構造登録者

Gabdulkhakov, A.G.,Tishchenko, T.V. (登録日: 2017-12-20, 公開日: 2019-01-30, 最終更新日: 2024-01-17)

主引用文献

Gabdulkhakov, A.,Kolyadenko, I.,Kostareva, O.,Mikhaylina, A.,Oliveira, P.,Tamagnini, P.,Lisov, A.,Tishchenko, S.
Investigations of Accessibility of T2/T3 Copper Center of Two-Domain Laccase fromStreptomyces griseoflavusAc-993.
Int J Mol Sci, 20:-, 2019

PubMed Abstract: Laccases (EC 1.10.3.2) are multicopper oxidoreductases acting on diphenols and related substances. Laccases are highly important for biotechnology and environmental remediation. These enzymes contain mononuclear one T2 copper ion and two T3 copper ions (Cu3 and Cu3), which form the so-called trinuclear center (TNC). Along with the typical three-domain laccases Bacteria produce two-domain (2D) enzymes, which are active at neutral and basic pH, thermostable, and resistant to inhibitors. In this work we present the comparative analysis of crystal structures and catalytic properties of recombinant 2D laccase from Ac-993 (SgfSL) and its four mutant forms with replacements of two amino acid residues, located at the narrowing of the presumable T3-solvent tunnels. We obtained inactive enzymes with substitutions of His165, with Phe, and Ile170 with Ala or Phe. His165Ala variant was more active than the wild type. We suggest that His165 is a "gateway" at the O-tunnel leading from solvent to the Cu3 of the enzyme. The side chain of Ile170 could be indirectly involved in the coordination of copper ions at the T3 center by maintaining the position of the imidazole ring of His157 that belongs to the first coordination sphere of Cu3.

PubMed: 31261802
DOI: 10.3390/ijms20133184

実験手法

X-RAY DIFFRACTION (1.95 Å)