6FB0

Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Calcium

Summary for 6FB0

• Entry DOI: 10.2210/pdb6fb0/pdb
• Descriptor: DNA endonuclease I-CreI, DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*CP*TP*CP*CP*AP*CP*AP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3'), DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*GP*AP*GP*AP*AP*GP*TP*CP*TP*GP*A)-3'), ... (8 entities in total)
• Functional Keywords: chlamydomonas reinhardtii, dna binding protein
• Biological source: Chlamydomonas reinhardtii (Chlamydomonas smithii); More
• Total number of polymer chains: 4
• Total formula weight: 50458.61

Authors

Molina, R.,Prieto, J. (deposition date: 2017-12-18, release date: 2018-07-18, Last modification date: 2024-01-17)

• Primary citation: Prieto, J.,Redondo, P.,Lopez-Mendez, B.,D'Abramo, M.,Merino, N.,Blanco, F.J.,Duchateau, P.,Montoya, G.,Molina, R.; Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.; Sci Rep, 8:10286-10286, 2018; PubMed: 29980759; DOI: 10.1038/s41598-018-28599-0

Experimental method

X-RAY DIFFRACTION (2.15 Å)