6F8H

antitoxin GraA

6F8H の概要

• エントリーDOI: 10.2210/pdb6f8h/pdb
• 分子名称: XRE family transcriptional regulator (2 entities in total)
• 機能のキーワード: graa, higa, antitoxin
• 由来する生物種: Pseudomonas putida (Arthrobacter siderocapsulatus)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 46985.60

構造登録者

Talavera, A.,Loris, R. (登録日: 2017-12-13, 公開日: 2019-01-30, 最終更新日: 2024-05-08)

主引用文献

Talavera, A.,Tamman, H.,Ainelo, A.,Konijnenberg, A.,Hadzi, S.,Sobott, F.,Garcia-Pino, A.,Horak, R.,Loris, R.
A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.
Nat Commun, 10:972-972, 2019

PubMed Abstract: Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth in favorable conditions or growth arrest during stress. A typical regulatory strategy involves the antitoxin binding and repressing its own promoter while the toxin often acts as a co-repressor. Here we show that Pseudomonas putida graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the sense that not the antitoxin but the toxin possesses a flexible region. GraA auto-represses the graTA promoter: two GraA dimers bind cooperatively at opposite sides of the operator sequence. Contrary to other TA modules, GraT is a de-repressor of the graTA promoter as its N-terminal disordered segment prevents the binding of the GraTA complex to the operator. Removal of this region restores operator binding and abrogates Gr aT toxicity. GraTA represents a TA module where a flexible region in the toxin rather than in the antitoxin controls operon expression and toxin activity.

PubMed: 30814507
DOI: 10.1038/s41467-019-08865-z

実験手法

X-RAY DIFFRACTION (2.002 Å)