6F6W

Structure of Mycobacterium smegmatis RNA polymerase core

6F6W の概要

• エントリーDOI: 10.2210/pdb6f6w/pdb
• 関連するPDBエントリー: 6EYD
• EMDBエントリー: 3983 4192
• 分子名称: DNA-directed RNA polymerase subunit alpha, DNA-directed RNA polymerase subunit beta, DNA-directed RNA polymerase subunit beta', ... (6 entities in total)
• 機能のキーワード: transcription sigma, transcription
• 由来する生物種: Mycolicibacterium smegmatis MC2 155; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 364829.59

構造登録者

Kouba, T.,Barvik, I.,Krasny, L. (登録日: 2017-12-06, 公開日: 2018-12-19, 最終更新日: 2024-05-15)

主引用文献

Kouba, T.,Pospisil, J.,Hnilicova, J.,Sanderova, H.,Barvik, I.,Krasny, L.
The Core and Holoenzyme Forms of RNA Polymerase fromMycobacterium smegmatis.
J. Bacteriol., 201:-, 2019

PubMed Abstract: Bacterial RNA polymerase (RNAP) is essential for gene expression and as such is a valid drug target. Hence, it is imperative to know its structure and dynamics. Here, we present two as-yet-unreported forms of RNAP: core and holoenzyme containing σ but no other factors. Each form was detected by cryo-electron microscopy in two major conformations. Comparisons of these structures with known structures of other RNAPs reveal a high degree of conformational flexibility of the mycobacterial enzyme and confirm that region 1.1 of σ is directed into the primary channel of RNAP. Taken together, we describe the conformational changes of unrestrained mycobacterial RNAP. We describe here three-dimensional structures of core and holoenzyme forms of mycobacterial RNA polymerase (RNAP) solved by cryo-electron microscopy. These structures fill the thus-far-empty spots in the gallery of the pivotal forms of mycobacterial RNAP and illuminate the extent of conformational dynamics of this enzyme. The presented findings may facilitate future designs of antimycobacterial drugs targeting RNAP.

PubMed: 30478083
DOI: 10.1128/JB.00583-18

実験手法

ELECTRON MICROSCOPY (3.81 Å)