6F60

The x-ray structure of bovine pancreatic ribonuclease in complex with a five-coordinate platinum(II) compound containing a sugar ligand

6F60 の概要

• エントリーDOI: 10.2210/pdb6f60/pdb
• 分子名称: Ribonuclease pancreatic, SULFATE ION, five-coordinate platinum(II) compound, ... (5 entities in total)
• 機能のキーワード: ribonuclease, platinum(ii) compound, five-coordinate, sugar ligand, cytotoxicity, hydrolase
• 由来する生物種: Bos taurus (Bovine)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28683.76

構造登録者

Merlino, A.,Ferraro, G. (登録日: 2017-12-04, 公開日: 2018-04-25, 最終更新日: 2024-11-20)

主引用文献

Cucciolito, M.E.,D'Amora, A.,De Feo, G.,Ferraro, G.,Giorgio, A.,Petruk, G.,Monti, D.M.,Merlino, A.,Ruffo, F.
Five-Coordinate Platinum(II) Compounds Containing Sugar Ligands: Synthesis, Characterization, Cytotoxic Activity, and Interaction with Biological Macromolecules.
Inorg Chem, 57:3133-3143, 2018

PubMed Abstract: This article describes the synthesis and characterization of novel cationic five-coordinate Pt(II) complexes containing nitrogen sugar-based ligands. The cytotoxicity of the complexes was evaluated on different cell lines with the expectation that both the coordinative saturation and the sugar moiety cooperate to enhance their biological activity. In fact, the complexes resulted to be more active than cisplatin but still with little selectivity. They activate the apoptosis pathway. Binding of representative compounds with DNA was studied by ethidium bromide displacement assay and circular dichroism. Binding to model proteins was also investigated; the X-ray structure of the adduct formed in the reaction between a representative compound and the model protein bovine pancreatic ribonuclease was obtained. The structure discloses an unprecedented interaction between a five-coordinate Pt(II) moiety and a His side chain.

PubMed: 29509011
DOI: 10.1021/acs.inorgchem.7b03118

実験手法

X-RAY DIFFRACTION (1.14 Å)