6F5F

Structure of ARTD2/PARP2 WGR domain bound to double strand DNA with 5 nucleotide overhang and 5'phosphate

6F5F の概要

• エントリーDOI: 10.2210/pdb6f5f/pdb
• 分子名称: Poly [ADP-ribose] polymerase 2, DNA (5'-D(P*CP*GP*GP*TP*CP*GP*CP*CP*TP*AP*TP*AP*GP*GP*C)-3') (2 entities in total)
• 機能のキーワード: adp-ribosylation, dna repair, dna end joining, artd2, non-phosphorylated dna, dna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 79444.61

構造登録者

Obaji, E.,Haikarainen, T.,Lehtio, L. (登録日: 2017-12-01, 公開日: 2018-10-10, 最終更新日: 2024-01-17)

主引用文献

Obaji, E.,Haikarainen, T.,Lehtio, L.
Structural basis for DNA break recognition by ARTD2/PARP2.
Nucleic Acids Res., 46:12154-12165, 2018

PubMed Abstract: Human ARTD2 (or PARP2) is an ADP-ribosyltransferase, which is catalytically activated by binding to damaged DNA. ARTD2 subsequently ADP-ribosylates itself and other proteins, initiating a cascade of events leading to DNA repair. In contrast to ARTD1, the founding member of the enzyme family, ARTD2 does not have specialized zinc-fingers for detecting DNA damage. The domain organization of ARTD2 includes disordered N-terminus, WGR and catalytic domains. However, the N-terminus of ARTD2 is not strictly required for the DNA dependent activity. While it is known that ARTD2 requires the WGR domain for efficient DNA binding and subsequent catalytic activation, the mechanism of DNA damage detection and subsequent catalytic activation are not completely understood. Here, we report crystal structures of ARTD2 WGR domain bound to double-strand break mimicking DNA oligonucleotides. Notably, the crystal structures revealed DNA binding mode of ARTD2 involving DNA end to end interaction. Structures demonstrate how ARTD2 recognizes nicked DNA, how it interacts with the 5'-phosphate group, and how it can mediate joining of DNA ends in vitro. Extensive mutagenesis of the ARTD2-DNA interface combined with activity, binding, and stoichiometry measurements demonstrate that the WGR domain is the key for DNA break detection.

PubMed: 30321391
DOI: 10.1093/nar/gky927

実験手法

X-RAY DIFFRACTION (2.98 Å)