6F4Z

2'F-araG modified quadruplex with flipped G-tract and central tetrad

Summary for 6F4Z

• Entry DOI: 10.2210/pdb6f4z/pdb
• NMR Information: BMRB: 34210
• Descriptor: DNA (5'-D(*GP*GP*GP*AP*TP*GP*GP*GP*AP*CP*AP*CP*AP*GP*(GFL)P*GP*GP*AP*CP*GP*GP*G)-3') (1 entity in total)
• Functional Keywords: g-quadruplex, dna, 2'f-ana
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 6990.48

Authors

Dickerhoff, J.,Weisz, K. (deposition date: 2017-11-30, release date: 2018-04-25, Last modification date: 2024-05-15)

Primary citation

Dickerhoff, J.,Weisz, K.
Fluorine-Mediated Editing of a G-Quadruplex Folding Pathway.
Chembiochem, 19:927-930, 2018

PubMed Abstract: A (3+1)-hybrid-type G-quadruplex was substituted within its central tetrad by a single 2'-fluoro-modified guanosine. Driven by the anti-favoring nucleoside analogue, a novel quadruplex fold with inversion of a single G-tract and conversion of a propeller loop into a lateral loop emerges. In addition, scalar couplings across hydrogen bonds demonstrate the formation of intra- and inter-residual F⋅⋅⋅H8-C8 pseudo-hydrogen bonds within the modified quadruplexes. Alternative folding can be rationalized by the impact of fluorine on intermediate species on the basis of a kinetic partitioning mechanism. Apparently, chemical or other environmental perturbations are able to redirect folding of a quadruplex, possibly modulating its regulatory role in physiological processes.

PubMed: 29460996
DOI: 10.1002/cbic.201800099

Experimental method

SOLUTION NMR