6F08

14-3-3 zeta in complex with the human Son of sevenless homolog 1 (SOS1)

Summary for 6F08

• Entry DOI: 10.2210/pdb6f08/pdb
• Descriptor: 14-3-3 protein zeta/delta, Son of sevenless homolog 1, PENTAETHYLENE GLYCOL, ... (4 entities in total)
• Functional Keywords: 14-3-3, sos1, dimer, phosphosite, peptide binding protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Cytoplasm : P63104
• Total number of polymer chains: 8
• Total formula weight: 111595.48

Authors

Ballone, A.,Centorrino, F.,Ottmann, C.,Guo, S.,Leysen, S. (deposition date: 2017-11-17, release date: 2018-02-14, Last modification date: 2024-10-09)

Primary citation

Ballone, A.,Centorrino, F.,Wolter, M.,Ottmann, C.
Structural characterization of 14-3-3 zeta in complex with the human Son of sevenless homolog 1 (SOS1).
J. Struct. Biol., 202:210-215, 2018

PubMed Abstract: The deviant Ras activation machinery is found in approximately 30% of all human cancers. SOS1 is an important protagonist of this pathway that plays a key-role in aberrant cell proliferation and differentiation. Interaction of SOS1 with 14-3-3 proteins modulates SOS1 activity in Ras-MAPK signaling. In the present study, we analyze the 14-3-3/SOS1 protein-protein interaction (PPI) by different biochemical assays and report the high resolution crystal structure of a 13-mer motif of SOS1 bound to 14-3-3ζ. These structural and functional insights are important for the evaluation of this PPI interface for small-molecule stabilization as a new starting point for modulating the Ras-Raf-MAPK pathway.

PubMed: 29408703
DOI: 10.1016/j.jsb.2018.01.011

Experimental method

X-RAY DIFFRACTION (1.9 Å)