6F08

14-3-3 zeta in complex with the human Son of sevenless homolog 1 (SOS1)

6F08 の概要

• エントリーDOI: 10.2210/pdb6f08/pdb
• 分子名称: 14-3-3 protein zeta/delta, Son of sevenless homolog 1, PENTAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: 14-3-3, sos1, dimer, phosphosite, peptide binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm : P63104
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 111595.48

構造登録者

Ballone, A.,Centorrino, F.,Ottmann, C.,Guo, S.,Leysen, S. (登録日: 2017-11-17, 公開日: 2018-02-14, 最終更新日: 2024-10-09)

主引用文献

Ballone, A.,Centorrino, F.,Wolter, M.,Ottmann, C.
Structural characterization of 14-3-3 zeta in complex with the human Son of sevenless homolog 1 (SOS1).
J. Struct. Biol., 202:210-215, 2018

PubMed Abstract: The deviant Ras activation machinery is found in approximately 30% of all human cancers. SOS1 is an important protagonist of this pathway that plays a key-role in aberrant cell proliferation and differentiation. Interaction of SOS1 with 14-3-3 proteins modulates SOS1 activity in Ras-MAPK signaling. In the present study, we analyze the 14-3-3/SOS1 protein-protein interaction (PPI) by different biochemical assays and report the high resolution crystal structure of a 13-mer motif of SOS1 bound to 14-3-3ζ. These structural and functional insights are important for the evaluation of this PPI interface for small-molecule stabilization as a new starting point for modulating the Ras-Raf-MAPK pathway.

PubMed: 29408703
DOI: 10.1016/j.jsb.2018.01.011

実験手法

X-RAY DIFFRACTION (1.9 Å)