6EOK

Crystal structure of E. coli L-asparaginase II

Summary for 6EOK

Entry DOI	10.2210/pdb6eok/pdb
Related	1IHD 1JAZ 1NNS 3ECA
Descriptor	L-asparaginase 2, ZINC ION (3 entities in total)
Functional Keywords	l-asparaginase ii, hydrolase
Biological source	Escherichia coli (strain K12)
Total number of polymer chains	4
Total formula weight	138768.87
Authors	Cerofolini, L.,Giuntini, S.,Carlon, A.,Ravera, E.,Calderone, V.,Fragai, M.,Parigi, G.,Luchinat, C. (deposition date: 2017-10-09, release date: 2018-10-31, Last modification date: 2024-11-06)
Primary citation	Cerofolini, L.,Giuntini, S.,Carlon, A.,Ravera, E.,Calderone, V.,Fragai, M.,Parigi, G.,Luchinat, C. Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics. Chemistry, 25:1984-1991, 2019 Cited by PubMed Abstract: Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography. PubMed: 30462348 DOI: 10.1002/chem.201804488 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation