6ENW

X-ray structure of Auoxo4-encapsulated horse spleen apoferritin

6ENW の概要

• エントリーDOI: 10.2210/pdb6enw/pdb
• 分子名称: Ferritin light chain, CADMIUM ION, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: drug delivery, nanocarrier, ferritin, gold, encapsulation, cytotoxicity, protein transport
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21610.11

構造登録者

Ferraro, G.,Merlino, A. (登録日: 2017-10-06, 公開日: 2017-12-13, 最終更新日: 2024-01-17)

主引用文献

Monti, D.M.,Ferraro, G.,Petruk, G.,Maiore, L.,Pane, F.,Amoresano, A.,Cinellu, M.A.,Merlino, A.
Ferritin nanocages loaded with gold ions induce oxidative stress and apoptosis in MCF-7 human breast cancer cells.
Dalton Trans, 46:15354-15362, 2017

PubMed Abstract: Two anticancer gold(iii) compounds, Au2phen and Auoxo4, were encapsulated within a ferritin nanocage. The gold-compound loaded proteins were characterized by UV-Vis spectroscopy, inductively coupled plasma mass spectrometry and circular dichroism. X-ray crystallography shows that the compounds degrade upon encapsulation and gold(i) ions bind Ft within the cage, close to the side chains of Cys126. The gold-encapsulated nanocarriers are cytotoxic to human cancer cells. Au(i)-loaded Ft, obtained upon the encapsulation of Au2phen within the cage, induces oxidative stress activation, which finally leads to apoptosis in MCF-7 cells.

PubMed: 29072740
DOI: 10.1039/c7dt02370g

実験手法

X-RAY DIFFRACTION (2.6 Å)