6ELK

C.elegans MnSOD-3 mutant - Q142H

Summary for 6ELK

• Entry DOI: 10.2210/pdb6elk/pdb
• Related: 3DC5 4X9Q
• Descriptor: Superoxide dismutase [Mn] 2, mitochondrial, MANGANESE (II) ION, SULFATE ION, ... (5 entities in total)
• Functional Keywords: superoxide dismutase, manganese, oxidoreductase
• Biological source: Caenorhabditis elegans
• Cellular location: Mitochondrion : P41977
• Total number of polymer chains: 2
• Total formula weight: 45517.34

Authors

Hunter, G.J.,Trinh, C.H.,Hunter, T. (deposition date: 2017-09-29, release date: 2017-12-06, Last modification date: 2024-01-17)

Primary citation

Hunter, T.,Bonetta, R.,Sacco, A.,Vella, M.,Sultana, P.M.,Trinh, C.H.,Fadia, H.B.R.,Borowski, T.,Garcia-Fandino, R.,Stockner, T.,Hunter, G.J.
A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron.
Chemistry, 24:5303-5308, 2018

PubMed Abstract: We have generated a site-directed mutant of the manganese superoxide dismutase SOD-3 of C.elegans (MnSOD-3) which modifies the metal specificity of the enzyme. While wild-type MnSOD-3 functions with manganese in the active site (3600 U mg of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50 % of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or iron.

PubMed: 29178484
DOI: 10.1002/chem.201704655

Experimental method

X-RAY DIFFRACTION (1.65 Å)