6EKT

Structure of P47 from Clostridium botulinum A2

Summary for 6EKT

• Entry DOI: 10.2210/pdb6ekt/pdb
• Descriptor: p-47 protein (2 entities in total)
• Functional Keywords: lipid binding, lipid binding protein
• Biological source: Clostridium botulinum (strain Kyoto / Type A2)
• Total number of polymer chains: 1
• Total formula weight: 50073.55

Authors

Berntsson, R.P.,Stenmark, P. (deposition date: 2017-09-27, release date: 2017-10-25, Last modification date: 2024-05-08)

Primary citation

Gustafsson, R.,Berntsson, R.P.,Martinez-Carranza, M.,El Tekle, G.,Odegrip, R.,Johnson, E.A.,Stenmark, P.
Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster.
FEBS Lett., 591:3781-3792, 2017

PubMed Abstract: Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.

PubMed: 29067689
DOI: 10.1002/1873-3468.12889

Experimental method

X-RAY DIFFRACTION (1.75 Å)