6EHS

E. coli Hydrogenase-2 chemically reduced structure

6EHS の概要

• エントリーDOI: 10.2210/pdb6ehs/pdb
• 関連するPDBエントリー: 6EHQ
• 分子名称: Hydrogenase-2 small chain, Hydrogenase-2 large chain, IRON/SULFUR CLUSTER, ... (9 entities in total)
• 機能のキーワード: hydrogen oxidation, membrane protein, nife hydrogenase, oxidoreductase
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell membrane; Peripheral membrane protein; Periplasmic side: P69741; Cell membrane; Peripheral membrane protein: P0ACE0
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 190308.38

構造登録者

Carr, S.B.,Evans, R.M.,Beaton, S.E.,Armstrong, F.A. (登録日: 2017-09-15, 公開日: 2018-04-18, 最終更新日: 2024-11-06)

主引用文献

Beaton, S.E.,Evans, R.M.,Finney, A.J.,Lamont, C.M.,Armstrong, F.A.,Sargent, F.,Carr, S.B.
The structure of hydrogenase-2 fromEscherichia coli: implications for H2-driven proton pumping.
Biochem. J., 475:1353-1370, 2018

PubMed Abstract: Under anaerobic conditions, is able to metabolize molecular hydrogen via the action of several [NiFe]-hydrogenase enzymes. Hydrogenase-2, which is typically present in cells at low levels during anaerobic respiration, is a periplasmic-facing membrane-bound complex that functions as a proton pump to convert energy from hydrogen (H) oxidation into a proton gradient; consequently, its structure is of great interest. Empirically, the complex consists of a tightly bound core catalytic module, comprising large (HybC) and small (HybO) subunits, which is attached to an Fe-S protein (HybA) and an integral membrane protein (HybB). To date, efforts to gain a more detailed picture have been thwarted by low native expression levels of Hydrogenase-2 and the labile interaction between HybOC and HybA/HybB subunits. In the present paper, we describe a new overexpression system that has facilitated the determination of high-resolution crystal structures of HybOC and, hence, a prediction of the quaternary structure of the HybOCAB complex.

PubMed: 29555844
DOI: 10.1042/BCJ20180053

実験手法

X-RAY DIFFRACTION (1.5 Å)