6EEC

Mycobacterium tuberculosis RNAP promoter unwinding intermediate complex with RbpA/CarD and AP3 promoter captured by Corallopyronin

6EEC の概要

• エントリーDOI: 10.2210/pdb6eec/pdb
• EMDBエントリー: 9037 9039 9041
• 分子名称: DNA-directed RNA polymerase subunit alpha, methyl [(1E,5R)-5-{(3E)-3-[(2E,4E,8R,9E,12E)-1,8-dihydroxy-2,5,9-trimethyltetradeca-2,4,9,12-tetraen-1-ylidene]-2,4-dioxo-3,4-d ihydro-2H-pyran-6-yl}hex-1-en-1-yl]carbamate, ZINC ION, ... (12 entities in total)
• 機能のキーワード: initiation, transcription bubble, closed clamp, open promoter complex, transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 510846.41

構造登録者

Darst, S.A.,Campbell, E.A.,Boyaci Selcuk, H.,Chen, J. (登録日: 2018-08-13, 公開日: 2018-11-21, 最終更新日: 2025-06-04)

主引用文献

Boyaci, H.,Chen, J.,Jansen, R.,Darst, S.A.,Campbell, E.A.
Structures of an RNA polymerase promoter melting intermediate elucidate DNA unwinding.
Nature, 565:382-385, 2019

PubMed Abstract: A key regulated step of transcription is promoter melting by RNA polymerase (RNAP) to form the open promoter complex. To generate the open complex, the conserved catalytic core of the RNAP combines with initiation factors to locate promoter DNA, unwind 12-14 base pairs of the DNA duplex and load the template-strand DNA into the RNAP active site. Formation of the open complex is a multi-step process during which transient intermediates of unknown structure are formed. Here we present cryo-electron microscopy structures of bacterial RNAP-promoter DNA complexes, including structures of partially melted intermediates. The structures show that late steps of promoter melting occur within the RNAP cleft, delineate key roles for fork-loop 2 and switch 2-universal structural features of RNAP-in restricting access of DNA to the RNAP active site, and explain why clamp opening is required to allow entry of single-stranded template DNA into the active site. The key roles of fork-loop 2 and switch 2 suggest a common mechanism for late steps in promoter DNA opening to enable gene expression across all domains of life.

PubMed: 30626968
DOI: 10.1038/s41586-018-0840-5

実験手法

ELECTRON MICROSCOPY (3.55 Å)