6E7H
Crystal structure of H5 hemagglutinin mutant Y161A from A/Viet Nam/1203/2004 H5N1 influenza virus in complex with 3'-GcLN
Summary for 6E7H
| Entry DOI | 10.2210/pdb6e7h/pdb |
| Descriptor | Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total) |
| Functional Keywords | influenza virus, hemagglutinin, ha, h5n1, a/viet nam/1203/2004, receptor specificity, viral protein |
| Biological source | Influenza A virus (A/Viet Nam/1203/2004(H5N1)) More |
| Total number of polymer chains | 6 |
| Total formula weight | 177176.45 |
| Authors | Tzarum, N.,Wilson, I.A. (deposition date: 2018-07-26, release date: 2019-06-19, Last modification date: 2024-10-16) |
| Primary citation | Broszeit, F.,Tzarum, N.,Zhu, X.,Nemanichvili, N.,Eggink, D.,Leenders, T.,Li, Z.,Liu, L.,Wolfert, M.A.,Papanikolaou, A.,Martinez-Romero, C.,Gagarinov, I.A.,Yu, W.,Garcia-Sastre, A.,Wennekes, T.,Okamatsu, M.,Verheije, M.H.,Wilson, I.A.,Boons, G.J.,de Vries, R.P. N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses. Cell Rep, 27:3284-3294.e6, 2019 Cited by PubMed Abstract: A species barrier for the influenza A virus is the differential expression of sialic acid, which can either be α2,3-linked for avians or α2,6-linked for human viruses. The influenza A virus hosts also express other species-specific sialic acid derivatives. One major modification at C-5 is N-glycolyl (NeuGc), instead of N-acetyl (NeuAc). N-glycolyl is mammalian specific and expressed in pigs and horses, but not in humans, ferrets, seals, or dogs. Hemagglutinin (HA) adaptation to either N-acetyl or N-glycolyl is analyzed on a sialoside microarray containing both α2,3- and α2,6-linkage modifications on biologically relevant N-glycans. Binding studies reveal that avian, human, and equine HAs bind either N-glycolyl or N-acetyl. Structural data on N-glycolyl binding HA proteins of both H5 and H7 origin describe this specificity. Neuraminidases can cleave N-glycolyl efficiently, and tissue-binding studies reveal strict species specificity. The exclusive manner in which influenza A viruses differentiate between N-glycolyl and N-acetyl is indicative of selection. PubMed: 31189111DOI: 10.1016/j.celrep.2019.05.048 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3.3 Å) |
Structure validation
Download full validation report
