6E7H

Crystal structure of H5 hemagglutinin mutant Y161A from A/Viet Nam/1203/2004 H5N1 influenza virus in complex with 3'-GcLN

6E7H の概要

• エントリーDOI: 10.2210/pdb6e7h/pdb
• 分子名称: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: influenza virus, hemagglutinin, ha, h5n1, a/viet nam/1203/2004, receptor specificity, viral protein
• 由来する生物種: Influenza A virus (A/Viet Nam/1203/2004(H5N1)); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 177176.45

構造登録者

Tzarum, N.,Wilson, I.A. (登録日: 2018-07-26, 公開日: 2019-06-19, 最終更新日: 2024-10-16)

主引用文献

Broszeit, F.,Tzarum, N.,Zhu, X.,Nemanichvili, N.,Eggink, D.,Leenders, T.,Li, Z.,Liu, L.,Wolfert, M.A.,Papanikolaou, A.,Martinez-Romero, C.,Gagarinov, I.A.,Yu, W.,Garcia-Sastre, A.,Wennekes, T.,Okamatsu, M.,Verheije, M.H.,Wilson, I.A.,Boons, G.J.,de Vries, R.P.
N-Glycolylneuraminic Acid as a Receptor for Influenza A Viruses.
Cell Rep, 27:3284-3294.e6, 2019

PubMed Abstract: A species barrier for the influenza A virus is the differential expression of sialic acid, which can either be α2,3-linked for avians or α2,6-linked for human viruses. The influenza A virus hosts also express other species-specific sialic acid derivatives. One major modification at C-5 is N-glycolyl (NeuGc), instead of N-acetyl (NeuAc). N-glycolyl is mammalian specific and expressed in pigs and horses, but not in humans, ferrets, seals, or dogs. Hemagglutinin (HA) adaptation to either N-acetyl or N-glycolyl is analyzed on a sialoside microarray containing both α2,3- and α2,6-linkage modifications on biologically relevant N-glycans. Binding studies reveal that avian, human, and equine HAs bind either N-glycolyl or N-acetyl. Structural data on N-glycolyl binding HA proteins of both H5 and H7 origin describe this specificity. Neuraminidases can cleave N-glycolyl efficiently, and tissue-binding studies reveal strict species specificity. The exclusive manner in which influenza A viruses differentiate between N-glycolyl and N-acetyl is indicative of selection.

PubMed: 31189111
DOI: 10.1016/j.celrep.2019.05.048

実験手法

X-RAY DIFFRACTION (3.3 Å)