6DS1

Crystal structure of Cj0485 dehydrogenase in complex with NADP+

6DS1 の概要

• エントリーDOI: 10.2210/pdb6ds1/pdb
• 分子名称: Putative oxidoreductase, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE, GLYCEROL, ... (5 entities in total)
• 機能のキーワード: dehydrogenase, oxidoreductase
• 由来する生物種: Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 125434.41

構造登録者

Pluvinage, B.,Boraston, A.B. (登録日: 2018-06-13, 公開日: 2019-07-10, 最終更新日: 2023-10-11)

主引用文献

Garber, J.M.,Nothaft, H.,Pluvinage, B.,Stahl, M.,Bian, X.,Porfirio, S.,Enriquez, A.,Butcher, J.,Huang, H.,Glushka, J.,Line, E.,Gerlt, J.A.,Azadi, P.,Stintzi, A.,Boraston, A.B.,Szymanski, C.M.
The gastrointestinal pathogen Campylobacter jejuni metabolizes sugars with potential help from commensal Bacteroides vulgatus.
Commun Biol, 3:2-2, 2020

PubMed Abstract: Although the gastrointestinal pathogen Campylobacter jejuni was considered asaccharolytic, >50% of sequenced isolates possess an operon for L-fucose utilization. In C. jejuni NCTC11168, this pathway confers L-fucose chemotaxis and competitive colonization advantages in the piglet diarrhea model, but the catabolic steps remain unknown. Here we solved the putative dehydrogenase structure, resembling FabG of Burkholderia multivorans. The C. jejuni enzyme, FucX, reduces L-fucose and D-arabinose in vitro and both sugars are catabolized by fuc-operon encoded enzymes. This enzyme alone confers chemotaxis to both sugars in a non-carbohydrate-utilizing C. jejuni strain. Although C. jejuni lacks fucosidases, the organism exhibits enhanced growth in vitro when co-cultured with Bacteroides vulgatus, suggesting scavenging may occur. Yet, when excess amino acids are available, C. jejuni prefers them to carbohydrates, indicating a metabolic hierarchy exists. Overall this study increases understanding of nutrient metabolism by this pathogen, and identifies interactions with other gut microbes.

PubMed: 31925306
DOI: 10.1038/s42003-019-0727-5

実験手法

X-RAY DIFFRACTION (2.119 Å)