6DOY

Crystal Structure of Bacillus Halodurans Ribonuclease H1 in Complex with an RNA/DNA Hybrid: Reaction in 2 mM Mg2+ and 200 mM Li+ for 120 s at 21 C

Summary for 6DOY

• Entry DOI: 10.2210/pdb6doy/pdb
• Group deposition: Cleavage of RNA by B. Halodurans Ribonuclease H1 (G_1001001)
• Descriptor: Ribonuclease H, RNA (5'-R(*AP*CP*AP*UP*CP*G)-3'), DNA (5'-D(*CP*GP*AP*TP*GP*T)-3'), ... (8 entities in total)
• Functional Keywords: protein-rna-dna complex, double helix, rna hydrolysis, in crystallo catalysis, metal dependent catalysis, monovalent cations, divalent cations, hydrolase, hydrolase-dna-rna complex, hydrolase/dna/rna
• Biological source: Bacillus halodurans; More
• Total number of polymer chains: 3
• Total formula weight: 21232.11

Authors

Samara, N.L.,Yang, W. (deposition date: 2018-06-09, release date: 2018-08-15, Last modification date: 2023-10-11)

• Primary citation: Samara, N.L.,Yang, W.; Cation trafficking propels RNA hydrolysis.; Nat. Struct. Mol. Biol., 25:715-721, 2018; PubMed: 30076410; DOI: 10.1038/s41594-018-0099-4

Experimental method

X-RAY DIFFRACTION (1.45 Å)