6DA1

ETS1 in complex with synthetic SRR mimic

6DA1 の概要

• エントリーDOI: 10.2210/pdb6da1/pdb
• 分子名称: Protein C-ets-1, serine-rich region (SRR) peptide, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: ets1, transcription factor, complex, autoinhibition, dna binding protein
• 由来する生物種: Mus musculus (Mouse); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 35234.14

構造登録者

Perez-Borrajero, C.,Okon, M.,Lin, C.S.,Scheu, K.,Murphy, M.E.P.,Graves, B.J.,McIntosh, L.P. (登録日: 2018-05-01, 公開日: 2019-01-16, 最終更新日: 2024-11-06)

主引用文献

Perez-Borrajero, C.,Lin, C.S.,Okon, M.,Scheu, K.,Graves, B.J.,Murphy, M.E.P.,McIntosh, L.P.
The Biophysical Basis for Phosphorylation-Enhanced DNA-Binding Autoinhibition of the ETS1 Transcription Factor.
J. Mol. Biol., 431:593-614, 2019

PubMed Abstract: The eukaryotic transcription factor ETS1 is regulated by an intrinsically disordered serine-rich region (SRR) that transiently associates with the adjacent ETS domain to inhibit DNA binding. In this study, we further elucidated the physicochemical basis for ETS1 autoinhibition by characterizing the interaction of its ETS domain with a series of synthetic peptides corresponding to the SRR. Binding is driven by the hydrophobic effect and enhanced electrostatically by phosphorylation of serines adjacent to aromatic residues in the amphipathic SRR. Structural characterization of the dynamic peptide/protein complex by NMR spectroscopy and X-ray crystallography revealed multiple modes of binding that lead to autoinhibition by synergistically blocking the DNA-binding interface of the ETS domain and stabilizing an appended helical inhibitory module against allosterically induced unfolding. Consistent with these conclusions, the SRR peptide does not interact with DNA-bound ETS1. In addition, we found that the ETS1 SRR phosphopeptide binds to distantly related PU.1 in vitro, indicating that autoinhibition exploits features of the ETS domain that are conserved across this family of transcription factors.

PubMed: 30597162
DOI: 10.1016/j.jmb.2018.12.011

実験手法

X-RAY DIFFRACTION (2.0001269892 Å)