6D8P

Ternary RsAgo Complex Containing Guide RNA Paired with Target DNA

Summary for 6D8P

Related6D8F 6D8A 6D95 6D92
DescriptorUncharacterized protein, RNA (5'-R(P*UP*UP*AP*CP*UP*GP*CP*AP*CP*AP*GP*GP*UP*GP*AP*CP*GP*A)-3'), DNA (5'-D(P*TP*CP*GP*TP*CP*AP*CP*CP*TP*GP*TP*GP*CP*AP*GP*TP*AP*AP*C)-3'), ... (8 entities in total)
Functional Keywordsrhodobacter sphaerodes argounaute (rsago); guide rna; target dna; rna-dna heteroduplex; non-canonical base pairs and bulges, rna binding protein
Biological sourceRhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
More
Total number of polymer chains6
Total molecular weight206312.33
Authors
Liu, Y.,Esyunina, D.,Olovnikov, I.,Teplova, M.,Patel, D.J. (deposition date: 2018-04-26, release date: 2018-07-25)
Primary citation
Liu, Y.,Esyunina, D.,Olovnikov, I.,Teplova, M.,Kulbachinskiy, A.,Aravin, A.A.,Patel, D.J.
Accommodation of Helical Imperfections in Rhodobacter sphaeroides Argonaute Ternary Complexes with Guide RNA and Target DNA.
Cell Rep, 24:453-462, 2018
PubMed: 29996105 (PDB entries with the same primary citation)
DOI: 10.1016/j.celrep.2018.06.021
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.1 Å)
?

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone0.23550.5%6.5%3.8%0.60MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution