6CY0

RNA octamer containing 2'-F, 4'-Cbeta-OMe U.

6CY0 の概要

• エントリーDOI: 10.2210/pdb6cy0/pdb
• 関連するPDBエントリー: 5VR4
• 分子名称: RNA (5'-R(*(CBV)P*GP*AP*AP*(UFB)P*UP*CP*G)-3') (2 entities in total)
• 機能のキーワード: rna, oligonucleotide, modified base
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 21099.79

構造登録者

Harp, J.M.,Egli, M. (登録日: 2018-04-04, 公開日: 2018-08-29, 最終更新日: 2024-03-13)

主引用文献

Harp, J.M.,Guenther, D.C.,Bisbe, A.,Perkins, L.,Matsuda, S.,Bommineni, G.R.,Zlatev, I.,Foster, D.J.,Taneja, N.,Charisse, K.,Maier, M.A.,Rajeev, K.G.,Manoharan, M.,Egli, M.
Structural basis for the synergy of 4'- and 2'-modifications on siRNA nuclease resistance, thermal stability and RNAi activity.
Nucleic Acids Res., 46:8090-8104, 2018

PubMed Abstract: Chemical modification is a prerequisite of oligonucleotide therapeutics for improved metabolic stability, uptake and activity, irrespective of their mode of action, i.e. antisense, RNAi or aptamer. Phosphate moiety and ribose C2'/O2' atoms are the most common sites for modification. Compared to 2'-O-substituents, ribose 4'-C-substituents lie in proximity of both the 3'- and 5'-adjacent phosphates. To investigate potentially beneficial effects on nuclease resistance we combined 2'-F and 2'-OMe with 4'-Cα- and 4'-Cβ-OMe, and 2'-F with 4'-Cα-methyl modification. The α- and β-epimers of 4'-C-OMe-uridine and the α-epimer of 4'-C-Me-uridine monomers were synthesized and incorporated into siRNAs. The 4'α-epimers affect thermal stability only minimally and show increased nuclease stability irrespective of the 2'-substituent (H, F, OMe). The 4'β-epimers are strongly destabilizing, but afford complete resistance against an exonuclease with the phosphate or phosphorothioate backbones. Crystal structures of RNA octamers containing 2'-F,4'-Cα-OMe-U, 2'-F,4'-Cβ-OMe-U, 2'-OMe,4'-Cα-OMe-U, 2'-OMe,4'-Cβ-OMe-U or 2'-F,4'-Cα-Me-U help rationalize these observations and point to steric and electrostatic origins of the unprecedented nuclease resistance seen with the chain-inverted 4'β-U epimer. We used structural models of human Argonaute 2 in complex with guide siRNA featuring 2'-F,4'-Cα-OMe-U or 2'-F,4'-Cβ-OMe-U at various sites in the seed region to interpret in vitro activities of siRNAs with the corresponding 2'-/4'-C-modifications.

PubMed: 30107495
DOI: 10.1093/nar/gky703

実験手法

X-RAY DIFFRACTION (2.398 Å)