6CY0
RNA octamer containing 2'-F, 4'-Cbeta-OMe U.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.012, 47.631, 100.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.602 - 2.398 |
R-factor | 0.2043 |
Rwork | 0.202 |
R-free | 0.24230 |
Structure solution method | SAD |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.13_2992) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.602 | 2.490 |
High resolution limit [Å] | 2.398 | 2.398 |
Rmerge | 0.198 | 0.433 |
Rpim | 0.058 | 0.129 |
Number of reflections | 9164 | 907 |
<I/σ(I)> | 11 | |
Completeness [%] | 97.4 | |
Redundancy | 21.6 | |
CC(1/2) | 0.991 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.5 mM RNA; 100 mM MES, 2.5 M ammonium sulfate, 10 mM magnesium chloride, |