6CFZ

Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface

6CFZ の概要

• エントリーDOI: 10.2210/pdb6cfz/pdb
• EMDBエントリー: 7446 7469
• 分子名称: Ask1, Spc34, Dad3, ... (10 entities in total)
• 機能のキーワード: dash, dam1 complex, ask1, dad1, dad2, dad3, dad4, dam1, duo1, hsk3, spc19, spc34, kinetochore, kinetochore-microtubule interface, chromosome segregation, cell-division, point-centromere yeast, nuclear protein
• 由来する生物種: Chaetomium thermophilum; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 116148.55

構造登録者

Jenni, S.,Harrison, S.C. (登録日: 2018-02-19, 公開日: 2018-05-02, 最終更新日: 2024-03-13)

主引用文献

Jenni, S.,Harrison, S.C.
Structure of the DASH/Dam1 complex shows its role at the yeast kinetochore-microtubule interface.
Science, 360:552-558, 2018

PubMed Abstract: Kinetochores connect mitotic-spindle microtubules with chromosomes, allowing microtubule depolymerization to pull chromosomes apart during anaphase while resisting detachment as the microtubule shortens. The heterodecameric DASH/Dam1 complex (DASH/Dam1c), an essential component of yeast kinetochores, assembles into a microtubule-encircling ring. The ring associates with rodlike Ndc80 complexes to organize the kinetochore-microtubule interface. We report the cryo-electron microscopy structure (at ~4.5-angstrom resolution) of a DASH/Dam1c ring and a molecular model of its ordered components, validated by evolutionary direct-coupling analysis. Integrating this structure with that of the Ndc80 complex and with published interaction data yields a molecular picture of kinetochore-microtubule attachment, including how flexible, C-terminal extensions of DASH/Dam1c subunits project and contact widely separated sites on the Ndc80 complex rod and how phosphorylation at previously identified sites might regulate kinetochore assembly.

PubMed: 29724956
DOI: 10.1126/science.aar6436

実験手法

ELECTRON MICROSCOPY (4.5 Å)