6CFB
Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti
Summary for 6CFB
| Entry DOI | 10.2210/pdb6cfb/pdb |
| NMR Information | BMRB: 30409 |
| Descriptor | barrettide A (1 entity in total) |
| Functional Keywords | structure from molmol, disulfide rich peptide, antifungal, marine sponge, antifungal protein |
| Biological source | Geodia barretti |
| Total number of polymer chains | 1 |
| Total formula weight | 3220.46 |
| Authors | Rosengren, K.J.,Carstens, B.B.,Clark, R.J.,Goransson, U. (deposition date: 2018-02-14, release date: 2018-03-21, Last modification date: 2024-11-06) |
| Primary citation | Carstens, B.B.,Rosengren, K.J.,Gunasekera, S.,Schempp, S.,Bohlin, L.,Dahlstrom, M.,Clark, R.J.,Goransson, U. Isolation, Characterization, and Synthesis of the Barrettides: Disulfide-Containing Peptides from the Marine Sponge Geodia barretti. J. Nat. Prod., 78:1886-1893, 2015 Cited by PubMed Abstract: Two disulfide-containing peptides, barrettides A (1) and B (2), from the cold-water marine sponge Geodia barretti are described. Those 31 amino acid residue long peptides were sequenced using mass spectrometry methods and structurally characterized using NMR spectroscopy. The structure of 1 was confirmed by total synthesis using the solid-phase peptide synthesis approach that was developed. The two peptides were found to differ only at a single position in their sequence. The three-dimensional structure of 1 revealed that these peptides possess a unique fold consisting of a long β-hairpin structure that is cross-braced by two disulfide bonds in a ladder-like arrangement. The peptides are amphipathic in nature with the hydrophobic and charged residues clustered on separate faces of the molecule. The barrettides were found not to inhibit the growth of either Escherichia coli or Staphylococcus aureus but displayed antifouling activity against barnacle larvae (Balanus improvisus) without lethal effects in the concentrations tested. PubMed: 26222779DOI: 10.1021/acs.jnatprod.5b00210 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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