6CBQ

Crystal structure of QscR bound to agonist S3

Summary for 6CBQ

• Entry DOI: 10.2210/pdb6cbq/pdb
• Related: 3szt
• Descriptor: LuxR family transcriptional regulator, (2S)-2-hexyl-N-[(3S)-2-oxooxolan-3-yl]decanamide (3 entities in total)
• Functional Keywords: luxr-type ahl receptor, pseudomonas aeruginosa qscr, transcription, transcription-agonist complex, transcription/agonist
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 2
• Total formula weight: 55249.32

Authors

Churchill, M.E.A.,Wysoczynski-Horita, C.L. (deposition date: 2018-02-05, release date: 2018-02-28, Last modification date: 2023-10-04)

Primary citation

Wysoczynski-Horita, C.L.,Boursier, M.E.,Hill, R.,Hansen, K.,Blackwell, H.E.,Churchill, M.E.A.
Mechanism of agonism and antagonism of the Pseudomonas aeruginosa quorum sensing regulator QscR with non-native ligands.
Mol. Microbiol., 108:240-257, 2018

PubMed Abstract: Pseudomonas aeruginosa is an opportunistic pathogen that uses the process of quorum sensing (QS) to coordinate the expression of many virulence genes. During quorum sensing, N-acyl-homoserine lactone (AHL) signaling molecules regulate the activity of three LuxR-type transcription factors, LasR, RhlR and QscR. To better understand P. aeruginosa QS signal reception, we examined the mechanism underlying the response of QscR to synthetic agonists and antagonists using biophysical and structural approaches. The structure of QscR bound to a synthetic agonist reveals a novel mode of ligand binding supporting a general mechanism for agonist activity. In turn, antagonists of QscR with partial agonist activity were found to destabilize and greatly impair QscR dimerization and DNA binding. These results highlight the diversity of LuxR-type receptor responses to small molecule agonists and antagonists and demonstrate the potential for chemical strategies for the selective targeting of individual QS systems.

PubMed: 29437248
DOI: 10.1111/mmi.13930

Experimental method

X-RAY DIFFRACTION (2.8 Å)