6C96

Cryo-EM structure of mouse TPC1 channel in the apo state

Summary for 6C96

• Entry DOI: 10.2210/pdb6c96/pdb
• EMDB information: 7434
• Descriptor: Two pore calcium channel protein 1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (4 entities in total)
• Functional Keywords: ion channel, membrane protein
• Biological source: Mus musculus (Mouse)
• Total number of polymer chains: 2
• Total formula weight: 192166.71

Authors

She, J.,Guo, J.,Chen, Q.,Bai, X.,Jiang, Y. (deposition date: 2018-01-25, release date: 2018-04-04, Last modification date: 2024-11-06)

Primary citation

She, J.,Guo, J.,Chen, Q.,Zeng, W.,Jiang, Y.,Bai, X.C.
Structural insights into the voltage and phospholipid activation of the mammalian TPC1 channel.
Nature, 556:130-134, 2018

PubMed Abstract: The organellar two-pore channel (TPC) functions as a homodimer, in which each subunit contains two homologous Shaker-like six-transmembrane (6-TM)-domain repeats. TPCs belong to the voltage-gated ion channel superfamily and are ubiquitously expressed in animals and plants. Mammalian TPC1 and TPC2 are localized at the endolysosomal membrane, and have critical roles in regulating the physiological functions of these acidic organelles. Here we present electron cryo-microscopy structures of mouse TPC1 (MmTPC1)-a voltage-dependent, phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P)-activated Na-selective channel-in both the apo closed state and ligand-bound open state. Combined with functional analysis, these structures provide comprehensive structural insights into the selectivity and gating mechanisms of mammalian TPC channels. The channel has a coin-slot-shaped ion pathway in the filter that defines the selectivity of mammalian TPCs. Only the voltage-sensing domain from the second 6-TM domain confers voltage dependence on MmTPC1. Endolysosome-specific PtdIns(3,5)P binds to the first 6-TM domain and activates the channel under conditions of depolarizing membrane potential. Structural comparisons between the apo and PtdIns(3,5)P-bound structures show the interplay between voltage and ligand in channel activation. These MmTPC1 structures reveal lipid binding and regulation in a 6-TM voltage-gated channel, which is of interest in light of the emerging recognition of the importance of phosphoinositide regulation of ion channels.

PubMed: 29562233
DOI: 10.1038/nature26139

Experimental method

ELECTRON MICROSCOPY (3.4 Å)