6C3O

Cryo-EM structure of human KATP bound to ATP and ADP in quatrefoil form

6C3O の概要

• エントリーDOI: 10.2210/pdb6c3o/pdb
• EMDBエントリー: 7338
• 分子名称: ATP-sensitive inward rectifier potassium channel 11, ATP-binding cassette sub-family C member 8, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: atp-dependent potassium channel kir6.2 sur1 atp adp, transport protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Membrane; Multi-pass membrane protein: Q14654; Cell membrane ; Multi-pass membrane protein : Q09428
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 895376.33

構造登録者

Lee, K.P.K.,Chen, J.,MacKinnon, R. (登録日: 2018-01-10, 公開日: 2018-01-31, 最終更新日: 2024-11-13)

主引用文献

Lee, K.P.K.,Chen, J.,MacKinnon, R.
Molecular structure of human KATP in complex with ATP and ADP
Elife, 6:-, 2017

PubMed Abstract: In many excitable cells, KATP channels respond to intracellular adenosine nucleotides: ATP inhibits while ADP activates. We present two structures of the human pancreatic KATP channel, containing the ABC transporter SUR1 and the inward-rectifier K channel Kir6.2, in the presence of Mg and nucleotides. These structures, referred to as quatrefoil and propeller forms, were determined by single-particle cryo-EM at 3.9 Å and 5.6 Å, respectively. In both forms, ATP occupies the inhibitory site in Kir6.2. The nucleotide-binding domains of SUR1 are dimerized with Mg-ATP in the degenerate site and Mg-ADP in the consensus site. A lasso extension forms an interface between SUR1 and Kir6.2 adjacent to the ATP site in the propeller form and is disrupted in the quatrefoil form. These structures support the role of SUR1 as an ADP sensor and highlight the lasso extension as a key regulatory element in ADP's ability to override ATP inhibition.

PubMed: 29286281
DOI: 10.7554/eLife.32481

実験手法

ELECTRON MICROSCOPY (3.9 Å)