6C1Y

mbd of human mecp2 in complex with methylated DNA

6C1Y の概要

• エントリーDOI: 10.2210/pdb6c1y/pdb
• 分子名称: Methyl-CpG-binding protein 2, 12-mer DNA, UNKNOWN ATOM OR ION (3 entities in total)
• 機能のキーワード: mbd, dna methylation, structural genomics, structural genomics consortium, sgc, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 34283.68

構造登録者

Liu, K.,Bian, C.,Tempel, W.,Wernimont, A.K.,Arrowsmith, C.H.,Bountra, C.,Edwards, A.M.,Min, J.,Structural Genomics Consortium (SGC) (登録日: 2018-01-05, 公開日: 2018-02-14, 最終更新日: 2023-10-04)

主引用文献

Lei, M.,Tempel, W.,Chen, S.,Liu, K.,Min, J.
Plasticity at the DNA recognition site of the MeCP2 mCG-binding domain.
Biochim Biophys Acta Gene Regul Mech, 1862:194409-194409, 2019

PubMed Abstract: MeCP2 is an abundant protein, involved in transcriptional repression by binding to CG and non-CG methylated DNA. However, MeCP2 might also function as a transcription activator as MeCP2 is found bound to sparsely methylated promoters of actively expressed genes. Furthermore, Attachment Region Binding Protein (ARBP), the chicken ortholog of MeCP2, has been reported to bind to Matrix/scaffold attachment regions (MARs/SARs) DNA with an unmethylated 5'-CAC/GTG-3' consensus sequence. In our previous study, although we have systemically measured the binding abilities of MBDs to unmethylated CAC/GTG DNA and the complex structures reveal that the MBD2-MBD (MBD of MBD2) binds to the unmethylated CAC/GTG DNA by recognizing the complementary GTG trinucleotide, how the MeCP2-MBD (MBD of MeCP2) recognizes the unmethylated CAC/GTG DNA, especially the MARs DNA, is still unclear. In this study, we investigated the binding characteristics of MeCP2 in recognizing unmethylated 5'-CAC/GTG-3' motif containing DNA by binding and structural studies. We found that MeCP2-MBD binds to MARs DNA with a comparable binding affinity to mCG DNA, and the MeCP2-CAC/GTG complex structure revealed that MeCP2 residues R111 and R133 form base-specific interactions with the GTG motif. For comparison, we also determined crystal structures of the MeCP2-MBD bound to mCG and mCAC/GTG DNA, respectively. Together, these crystal structures illustrate the adaptability of the MeCP2-MBD toward the GTG motif as well as the mCG DNA, and also provide structural basis of a biological role of MeCP2 as a transcription activator and its disease implications in Rett syndrome.

PubMed: 31356990
DOI: 10.1016/j.bbagrm.2019.194409

実験手法

X-RAY DIFFRACTION (2.3 Å)