6BI7

Crystal structure of Rev7-WT/Rev3 as a monomer under high-salt conditions

6BI7 の概要

• エントリーDOI: 10.2210/pdb6bi7/pdb
• 関連するPDBエントリー: 6BC8 6BCD
• 分子名称: Mitotic spindle assembly checkpoint protein MAD2B, DNA polymerase zeta catalytic subunit (3 entities in total)
• 機能のキーワード: dna damage tolerance, translesion dna synthesis, replication
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 117861.42

構造登録者

Rizzo, A.A.,Korzhnev, D.M.,Hao, B.,Li, Y. (登録日: 2017-11-01, 公開日: 2018-08-01, 最終更新日: 2023-10-04)

主引用文献

Rizzo, A.A.,Vassel, F.M.,Chatterjee, N.,D'Souza, S.,Li, Y.,Hao, B.,Hemann, M.T.,Walker, G.C.,Korzhnev, D.M.
Rev7 dimerization is important for assembly and function of the Rev1/Pol zeta translesion synthesis complex.
Proc. Natl. Acad. Sci. U.S.A., 115:E8191-E8200, 2018

PubMed Abstract: The translesion synthesis (TLS) polymerases Polζ and Rev1 form a complex that enables replication of damaged DNA. The Rev7 subunit of Polζ, which is a multifaceted HORMA (Hop1, Rev7, Mad2) protein with roles in TLS, DNA repair, and cell-cycle control, facilitates assembly of this complex by binding Rev1 and the catalytic subunit of Polζ, Rev3. Rev7 interacts with Rev3 by a mechanism conserved among HORMA proteins, whereby an open-to-closed transition locks the ligand underneath the "safety belt" loop. Dimerization of HORMA proteins promotes binding and release of this ligand, as exemplified by the Rev7 homolog, Mad2. Here, we investigate the dimerization of Rev7 when bound to the two Rev7-binding motifs (RBMs) in Rev3 by combining in vitro analyses of Rev7 structure and interactions with a functional assay in a Rev7 cell line. We demonstrate that Rev7 uses the conventional HORMA dimerization interface both to form a homodimer when tethered by the two RBMs in Rev3 and to heterodimerize with other HORMA domains, Mad2 and p31 Structurally, the Rev7 dimer can bind only one copy of Rev1, revealing an unexpected Rev1/Polζ architecture. In cells, mutation of the Rev7 dimer interface increases sensitivity to DNA damage. These results provide insights into the structure of the Rev1/Polζ TLS assembly and highlight the function of Rev7 homo- and heterodimerization.

PubMed: 30111544
DOI: 10.1073/pnas.1801149115

実験手法

X-RAY DIFFRACTION (2.8 Å)