6BCB

A Complex between PH Domain of p114RhoGEF and Activated RhoA Bound to a GTP Analog

6BCB の概要

• エントリーDOI: 10.2210/pdb6bcb/pdb
• 分子名称: Transforming protein RhoA, Rho guanine nucleotide exchange factor 18, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: rho gtpase guanine nucleotide exchange factors rhogef pleckstrin homology ph domain, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38272.02

構造登録者

Sternweis, P.C.,Chen, Z. (登録日: 2017-10-20, 公開日: 2017-12-13, 最終更新日: 2024-03-13)

主引用文献

Chen, Z.,Gutowski, S.,Sternweis, P.C.
Crystal structures of the PH domains from Lbc family of RhoGEFs bound to activated RhoA GTPase.
Data Brief, 17:356-362, 2018

PubMed Abstract: The Pleckstrin homology (PH) domains from the Lbc family of Rho Guanine Nucleotide Exchange Factors (Lbc RhoGEFs) interact with activated Rho family GTPases. All 7 Lbc RhoGEFs associate directly with activated Rho GTPases via their PH domains. However, the binding affinities between the PH domains and the GTPases vary greatly. Here we present two crystal structures at resolutions of 1.4 Å and 2.0 Å of RhoA complexed with the PH domain from p114RhoGEF (PDB access code 6BCB) and AKAP-LbcRhoGEF (PDB access code 6BCA), respectively. These high resolution structures, together with the earlier structures of PDZRhoGEF-PH·RhoA and p190RhoGEF-PH·RhoA complexes, identify a highly conserved interface between the PH domains from Lbc-RhoGEFs and activated Rho GTPases. This manuscript is related to the manuscript titled "Direct Regulation of p190RhoGEF by Activated Rho and Rac GTPases" published in the Journal of Structural Biology.

PubMed: 29876405
DOI: 10.1016/j.dib.2018.01.024

実験手法

X-RAY DIFFRACTION (1.401 Å)