6B9O

Structure of GH 38 Jack Bean alpha-mannosidase

6B9O の概要

• エントリーDOI: 10.2210/pdb6b9o/pdb
• 分子名称: Alpha-mannosidase from Canavalia ensiformis (jack bean), alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: mannosidase, plant protein, hydrolase
• 由来する生物種: Canavalia ensiformis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 227554.46

構造登録者

Howard, E.,Cousido-Siah, A.,Lepage, M.,Bodlenner, A.,Mitschler, A.,Meli, A.,De Riccardis, F.,Izzo, I.,Podjarny, A.,Compain, P. (登録日: 2017-10-11, 公開日: 2018-09-26, 最終更新日: 2024-11-20)

主引用文献

Howard, E.,Cousido-Siah, A.,Lepage, M.L.,Schneider, J.P.,Bodlenner, A.,Mitschler, A.,Meli, A.,Izzo, I.,Alvarez, H.A.,Podjarny, A.,Compain, P.
Structural Basis of Outstanding Multivalent Effects in Jack Bean alpha-Mannosidase Inhibition.
Angew. Chem. Int. Ed. Engl., 57:8002-8006, 2018

PubMed Abstract: Multivalent design of glycosidase inhibitors is a promising strategy for the treatment of diseases involving enzymatic hydrolysis of glycosidic bonds in carbohydrates. An essential prerequisite for successful applications is the atomic-level understanding of how outstanding binding enhancement occurs with multivalent inhibitors. Herein we report the first high-resolution crystal structures of the Jack bean α-mannosidase (JBα-man) in apo and inhibited states. The three-dimensional structure of JBα-man in complex with the multimeric cyclopeptoid-based inhibitor displaying the largest binding enhancements reported so far provides decisive insight into the molecular mechanisms underlying multivalent effects in glycosidase inhibition.

PubMed: 29722924
DOI: 10.1002/anie.201801202

実験手法

X-RAY DIFFRACTION (1.841 Å)