6B6E

Beta-Lactamase, mixed with Ceftriaxone, needles crystal form, 500ms

Summary for 6B6E

• Entry DOI: 10.2210/pdb6b6e/pdb
• Related: 6b6b 6b6c 6b6d
• Descriptor: Beta-lactamase, (2R)-2-[(1S)-1-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}-2-hydroxyethyl]-5-methylidene-5,6-dihydro-2H-1,3-thiazine-4-carboxylic acid, Ceftriaxone, ... (4 entities in total)
• Functional Keywords: beta-lactamase, antibiotic, hydrolase, hydrolase-antibiotic complex, hydrolase/antibiotic
• Biological source: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
• Total number of polymer chains: 1
• Total formula weight: 29354.88

Authors

Pandey, S.,Schmidt, M. (deposition date: 2017-10-01, release date: 2018-06-27, Last modification date: 2024-03-13)

Primary citation

Olmos, J.L.,Pandey, S.,Martin-Garcia, J.M.,Calvey, G.,Katz, A.,Knoska, J.,Kupitz, C.,Hunter, M.S.,Liang, M.,Oberthuer, D.,Yefanov, O.,Wiedorn, M.,Heyman, M.,Holl, M.,Pande, K.,Barty, A.,Miller, M.D.,Stern, S.,Roy-Chowdhury, S.,Coe, J.,Nagaratnam, N.,Zook, J.,Verburgt, J.,Norwood, T.,Poudyal, I.,Xu, D.,Koglin, J.,Seaberg, M.H.,Zhao, Y.,Bajt, S.,Grant, T.,Mariani, V.,Nelson, G.,Subramanian, G.,Bae, E.,Fromme, R.,Fung, R.,Schwander, P.,Frank, M.,White, T.A.,Weierstall, U.,Zatsepin, N.,Spence, J.,Fromme, P.,Chapman, H.N.,Pollack, L.,Tremblay, L.,Ourmazd, A.,Phillips, G.N.,Schmidt, M.
Enzyme intermediates captured "on the fly" by mix-and-inject serial crystallography.
BMC Biol., 16:59-59, 2018

PubMed Abstract: Ever since the first atomic structure of an enzyme was solved, the discovery of the mechanism and dynamics of reactions catalyzed by biomolecules has been the key goal for the understanding of the molecular processes that drive life on earth. Despite a large number of successful methods for trapping reaction intermediates, the direct observation of an ongoing reaction has been possible only in rare and exceptional cases.

PubMed: 29848358
DOI: 10.1186/s12915-018-0524-5

Experimental method

X-RAY DIFFRACTION (1.901 Å)