6B31

Structure of RORgt in complex with a novel inverse agonist 2

6B31 の概要

• エントリーDOI: 10.2210/pdb6b31/pdb
• 分子名称: Nuclear receptor ROR-gamma, (3S)-N~1~-(3-chloro-4-cyanophenyl)-N~5~-(1,3-diethyl-2,4-dioxo-1,2,3,4-tetrahydroquinazolin-6-yl)-3-methylpentanediamide (3 entities in total)
• 機能のキーワード: complex, inverse agonist, nuclear hormone receptor, signaling protein, signaling protein-agonist complex, signaling protein/agonist
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 54223.58

構造登録者

Skene, R.J.,Hoffman, I. (登録日: 2017-09-20, 公開日: 2018-08-01, 最終更新日: 2024-03-13)

主引用文献

Fukase, Y.,Sato, A.,Tomata, Y.,Ochida, A.,Kono, M.,Yonemori, K.,Koga, K.,Okui, T.,Yamasaki, M.,Fujitani, Y.,Nakagawa, H.,Koyama, R.,Nakayama, M.,Skene, R.,Sang, B.C.,Hoffman, I.,Shirai, J.,Yamamoto, S.
Identification of novel quinazolinedione derivatives as ROR gamma t inverse agonist.
Bioorg. Med. Chem., 26:721-736, 2018

PubMed Abstract: Novel small molecules were synthesized and evaluated as retinoic acid receptor-related orphan receptor-gamma t (RORγt) inverse agonists for the treatment of inflammatory and autoimmune diseases. A hit compound, 1, was discovered by high-throughput screening of our compound library. The structure-activity relationship (SAR) study of compound 1 showed that the introduction of a chlorine group at the 3-position of 4-cyanophenyl moiety increased the potency and a 3-methylpentane-1,5-diamide linker is favorable for the activity. The carbazole moiety of 1 was also optimized; a quinazolinedione derivative 18i suppressed the increase of IL-17A mRNA level in the lymph node of a rat model of experimental autoimmune encephalomyelitis (EAE) upon oral administration. These results indicate that the novel quinazolinedione derivatives have great potential as orally available small-molecule RORγt inverse agonists for the treatment of Th17-driven autoimmune diseases. A U-shaped bioactive conformation of this chemotype with RORγt protein was also observed.

PubMed: 29342416
DOI: 10.1016/j.bmc.2017.12.039

実験手法

X-RAY DIFFRACTION (3.18 Å)