6AZC
Crystal structure of Physcomitrella patens KAI2-like E S166A
Summary for 6AZC
| Entry DOI | 10.2210/pdb6azc/pdb |
| Descriptor | Pp-KAI2-like E (2 entities in total) |
| Functional Keywords | alpha/beta hydrolase, strigolactone binding, hydrolase |
| Biological source | Physcomitrella patens subsp. patens (Moss) |
| Total number of polymer chains | 2 |
| Total formula weight | 59758.23 |
| Authors | Burger, M.,Lee, H.J.,Chory, J. (deposition date: 2017-09-11, release date: 2019-02-06, Last modification date: 2023-10-04) |
| Primary citation | Burger, M.,Mashiguchi, K.,Lee, H.J.,Nakano, M.,Takemoto, K.,Seto, Y.,Yamaguchi, S.,Chory, J. Structural Basis of Karrikin and Non-natural Strigolactone Perception in Physcomitrella patens. Cell Rep, 26:855-865.e5, 2019 Cited by PubMed Abstract: In plants, strigolactones are perceived by the dual receptor-hydrolase DWARF14 (D14). D14 belongs to the superfamily of α/β hydrolases and is structurally similar to the karrikin receptor KARRIKIN INSENSITIVE 2 (KAI2). The moss Physcomitrella patens is an ideal model system for studying this receptor family, because it includes 11 highly related family members with unknown ligand specificity. We present the crystal structures of three Physcomitrella D14/KAI2-like proteins and describe a loop-based mechanism that leads to a permanent widening of the hydrophobic substrate gorge. We have identified protein clades that specifically perceive the karrikin KAR and the non-natural strigolactone isomer (-)-5-deoxystrigol in a highly stereoselective manner. PubMed: 30673608DOI: 10.1016/j.celrep.2019.01.003 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.00001213332 Å) |
Structure validation
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