6AQX

Crystal Structure of Z-DNA with 6-fold Twinning_Z4B

Summary for 6AQX

• Entry DOI: 10.2210/pdb6aqx/pdb
• Descriptor: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') (2 entities in total)
• Functional Keywords: z-dna, twinning, dna
• Biological source: synthetic construct
• Total number of polymer chains: 8
• Total formula weight: 14481.64

Authors

Luo, Z.,Dauter, Z.,Gilski, M. (deposition date: 2017-08-21, release date: 2017-08-30, Last modification date: 2023-10-04)

Primary citation

Luo, Z.,Dauter, Z.,Gilski, M.
Four highly pseudosymmetric and/or twinned structures of d(CGCGCG)2 extend the repertoire of crystal structures of Z-DNA.
Acta Crystallogr D Struct Biol, 73:940-951, 2017

PubMed Abstract: DNA oligomer duplexes containing alternating cytosines and guanines in their sequences tend to form left-handed helices of the Z-DNA type, with the sugar and phosphate backbone in a zigzag conformation and a helical repeat of two successive nucleotides. Z-DNA duplexes usually crystallize as hexagonally arranged parallel helical tubes, with various relative orientations and translation of neighboring duplexes. Four novel high-resolution crystal structures of d(CGCGCG) duplexes are described here. They are characterized by a high degree of pseudosymmetry and/or twinning, with three or four independent duplexes differently oriented in a monoclinic P2 lattice of hexagonal metric. The various twinning criteria give somewhat conflicting indications in these complicated cases of crystal pathology. The details of molecular packing in these crystal structures are compared with other known crystal forms of Z-DNA.

PubMed: 29095165
DOI: 10.1107/S2059798317014954

Experimental method

X-RAY DIFFRACTION (1.55 Å)