6AGF

Structure of the human voltage-gated sodium channel Nav1.4 in complex with beta1

6AGF の概要

• エントリーDOI: 10.2210/pdb6agf/pdb
• EMDBエントリー: 9617
• 分子名称: Sodium channel protein type 4 subunit alpha, Sodium channel subunit beta-1, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: sodium channel, membrane protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 244299.03

構造登録者

Pan, X.J.,li, Z.Q.,Zhou, Q.,Shen, H.Z.,Wu, K.,Huang, X.S.,Chen, J.F.,Zhang, J.R.,Zhu, X.C.,Lei, J.L.,Xiong, W.,Gong, H.P.,Xiao, B.L.,Yan, N. (登録日: 2018-08-11, 公開日: 2018-10-10, 最終更新日: 2024-11-20)

主引用文献

Pan, X.,Li, Z.,Zhou, Q.,Shen, H.,Wu, K.,Huang, X.,Chen, J.,Zhang, J.,Zhu, X.,Lei, J.,Xiong, W.,Gong, H.,Xiao, B.,Yan, N.
Structure of the human voltage-gated sodium channel Nav1.4 in complex with beta 1.
Science, 362:-, 2018

PubMed Abstract: Voltage-gated sodium (Na) channels, which are responsible for action potential generation, are implicated in many human diseases. Despite decades of rigorous characterization, the lack of a structure of any human Na channel has hampered mechanistic understanding. Here, we report the cryo-electron microscopy structure of the human Na1.4-β1 complex at 3.2-Å resolution. Accurate model building was made for the pore domain, the voltage-sensing domains, and the β1 subunit, providing insight into the molecular basis for Na permeation and kinetic asymmetry of the four repeats. Structural analysis of reported functional residues and disease mutations corroborates an allosteric blocking mechanism for fast inactivation of Na channels. The structure provides a path toward mechanistic investigation of Na channels and drug discovery for Na channelopathies.

PubMed: 30190309
DOI: 10.1126/science.aau2486

実験手法

ELECTRON MICROSCOPY (3.2 Å)