6T8F

Crystal structure of mutant xylose isomerase (V270A/A273G) from Piromyces E2 grown in yeast, in complex with xylose

Summary for 6T8F

Entry DOI	10.2210/pdb6t8f/pdb
Related	6T8E
Descriptor	Xylose isomerase, CALCIUM ION, D-xylose, ... (7 entities in total)
Functional Keywords	isomerase, tim-barrel
Biological source	Piromyces sp. (strain E2)
Total number of polymer chains	4
Total formula weight	201265.72
Authors	Rozeboom, H.J.,Janssen, D.B. (deposition date: 2019-10-24, release date: 2020-01-29, Last modification date: 2024-01-24)
Primary citation	Lee, M.,Rozeboom, H.J.,Keuning, E.,de Waal, P.,Janssen, D.B. Structure-based directed evolution improves S. cerevisiae growth on xylose by influencing in vivo enzyme performance. Biotechnol Biofuels, 13:5-5, 2020 Cited by PubMed Abstract: Efficient bioethanol production from hemicellulose feedstocks by requires xylose utilization. Whereas does not metabolize xylose, engineered strains that express xylose isomerase can metabolize xylose by converting it to xylulose. For this, the type II xylose isomerase from (PirXI) is used but the in vivo activity is rather low and very high levels of the enzyme are needed for xylose metabolism. In this study, we explore the use of protein engineering and in vivo selection to improve the performance of PirXI. Recently solved crystal structures were used to focus mutagenesis efforts. PubMed: 31938040 DOI: 10.1186/s13068-019-1643-0 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2 Å)

Structure validation