6T8F
Crystal structure of mutant xylose isomerase (V270A/A273G) from Piromyces E2 grown in yeast, in complex with xylose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Xylose isomerase | polymer | 437 | 49416.6 | 4 | UniProt (Q9P8C9) Pfam (PF01261) | Piromyces sp. (strain E2) | |
| 2 | BA, CA, E, F, M... (D, A, B, C) | CALCIUM ION | non-polymer | 40.1 | 8 | Chemie (CA) | |||
| 3 | DA, G, O, W (D, A, B, C) | D-xylose | non-polymer | 150.1 | 4 | Chemie (XLS) | |||
| 4 | EA, H, HA, P, X... (D, A, B, C) | beta-D-xylopyranose | non-polymer | 150.1 | 6 | Chemie (XYP) | |||
| 5 | FA, GA, I, J, Q... (D, A, B, C) | alpha-D-xylopyranose | non-polymer | 150.1 | 8 | Chemie (XYS) | |||
| 6 | AA, IA, JA, K, L... (C, D, A, B) | SULFATE ION | non-polymer | 96.1 | 6 | Chemie (SO4) | |||
| 7 | KA, LA, MA, NA (A, B, C, D) | water | water | 18.0 | 1721 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 437 (UniProt: Q9P8C9)
| PDB | External Database | Details |
|---|---|---|
| Ala 270 | Val 270 | engineered mutation |
| Gly 273 | Ala 273 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 197666.4 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 3599.3 | |
| All* | Total formula weight | 201265.7 |
