6NSC

Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin G186V/L194P mutant apo form

Summary for 6NSC

• Entry DOI: 10.2210/pdb6nsc/pdb
• Descriptor: Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• Functional Keywords: viral protein
• Biological source: Influenza A virus (A/Brisbane/10/2007(H3N2)); More
• Total number of polymer chains: 2
• Total formula weight: 58645.27

Authors

Wu, N.C.,Wilson, I.A. (deposition date: 2019-01-24, release date: 2019-04-24, Last modification date: 2023-10-11)

Primary citation

Wu, N.C.,Lv, H.,Thompson, A.J.,Wu, D.C.,Ng, W.W.S.,Kadam, R.U.,Lin, C.W.,Nycholat, C.M.,McBride, R.,Liang, W.,Paulson, J.C.,Mok, C.K.P.,Wilson, I.A.
Preventing an Antigenically Disruptive Mutation in Egg-Based H3N2 Seasonal Influenza Vaccines by Mutational Incompatibility.
Cell Host Microbe, 25:836-, 2019

PubMed Abstract: Egg-based seasonal influenza vaccines are the major preventive countermeasure against influenza virus. However, their effectiveness can be compromised when antigenic changes arise from egg-adaptive mutations on influenza hemagglutinin (HA). The L194P mutation is commonly observed in egg-based H3N2 vaccine seed strains and significantly alters HA antigenicity. An approach to prevent L194P would therefore be beneficial. We show that emergence of L194P during egg passaging can be impeded by preexistence of a G186V mutation, revealing strong incompatibility between these mutations. X-ray structures illustrate that individual G186V and L194P mutations have opposing effects on the HA receptor-binding site (RBS), and when both G186V and L194P are present, the RBS is severely disrupted. Importantly, wild-type HA antigenicity is maintained with G186V, but not L194P. Our results demonstrate that these epistatic interactions can be used to prevent the emergence of mutations that adversely alter antigenicity during egg adaptation.

PubMed: 31151913
DOI: 10.1016/j.chom.2019.04.013

Experimental method

X-RAY DIFFRACTION (2.25 Å)