6GWF

Alpha-galactosidase mutant D387A from Thermotoga maritima in complex with intact cyclohexene-based carbasugar mimic of galactose with 2,4-dinitro leaving group

Summary for 6GWF

Entry DOI	10.2210/pdb6gwf/pdb
Related	6GTA 6GVD
Descriptor	Alpha-galactosidase, SULFATE ION, (1~{S},2~{S},5~{S},6~{R})-5-(2,4-dinitrophenoxy)-6-fluoranyl-3-(hydroxymethyl)cyclohex-3-ene-1,2-diol, ... (5 entities in total)
Functional Keywords	glycoside hydrolase, galactosidase, carbohydrate processing enzyme, inhibitor, hydrolase
Biological source	Thermotoga maritima MSB8
Total number of polymer chains	1
Total formula weight	66714.77
Authors	Gloster, T.M.,Oehler, V. (deposition date: 2018-06-24, release date: 2018-08-22, Last modification date: 2024-01-17)
Primary citation	Ren, W.,Pengelly, R.,Farren-Dai, M.,Shamsi Kazem Abadi, S.,Oehler, V.,Akintola, O.,Draper, J.,Meanwell, M.,Chakladar, S.,Swiderek, K.,Moliner, V.,Britton, R.,Gloster, T.M.,Bennet, A.J. Revealing the mechanism for covalent inhibition of glycoside hydrolases by carbasugars at an atomic level. Nat Commun, 9:3243-3243, 2018 Cited by PubMed: 30104598 DOI: 10.1038/s41467-018-05702-7 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.72 Å)

Structure validation