6GWF

Alpha-galactosidase mutant D387A from Thermotoga maritima in complex with intact cyclohexene-based carbasugar mimic of galactose with 2,4-dinitro leaving group

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004557	molecular_function	alpha-galactosidase activity
A	0005975	biological_process	carbohydrate metabolic process
A	0016139	biological_process	glycoside catabolic process
A	0030246	molecular_function	carbohydrate binding
A	0042803	molecular_function	protein homodimerization activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue SO4 A 601

Chain	Residue
A	ARG9
A	GLY393
A	GLU394
A	HOH727
A	HOH753
A	HOH766
A	HOH824
A	HOH922

---

site_id	AC2
Number of Residues	4
Details	binding site for residue SO4 A 602

Chain	Residue
A	ASP303
A	GLU304
A	LYS339
A	LYS302

---

site_id	AC3
Number of Residues	19
Details	binding site for residue FEQ A 603

Chain	Residue
A	SER-4
A	ALA-1
A	ARG-6
A	GLY-5
A	TRP65
A	TRP190
A	TYR191
A	ASP220
A	ASP221
A	TRP257
A	LYS325
A	ASP327
A	PHE328
A	CYS368
A	ARG383
A	ALA387
A	HOH752
A	HOH983
A	HOH1056

---

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 604

Chain	Residue
A	ASP419
A	ASP454
A	HOH736
A	HOH933
A	HOH960
A	HOH971

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"17323919","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"17323919","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PubMed","id":"27783466","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5M16","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

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