6ANV

Crystal structure of anti-CRISPR protein AcrF1

Summary for 6ANV

• Entry DOI: 10.2210/pdb6anv/pdb
• Related PRD ID: PRD_900010
• Descriptor: anti-CRISPR protein AcrF1 fused with C-terminal MBP tag, alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, ... (7 entities in total)
• Functional Keywords: type i-f crispr-cas system: csy cascade: structure: anti-crispr protein: inhibition of csy complex: genome editing tool, immune system
• Biological source: Pseudomonas phage JBD30; More
• Total number of polymer chains: 2
• Total formula weight: 102897.75

Authors

Yang, H.,Patel, D.J. (deposition date: 2017-08-14, release date: 2017-10-25, Last modification date: 2023-10-04)

Primary citation

Guo, T.W.,Bartesaghi, A.,Yang, H.,Falconieri, V.,Rao, P.,Merk, A.,Eng, E.T.,Raczkowski, A.M.,Fox, T.,Earl, L.A.,Patel, D.J.,Subramaniam, S.
Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex.
Cell, 171:414-426.e12, 2017

PubMed Abstract: Prokaryotic cells possess CRISPR-mediated adaptive immune systems that protect them from foreign genetic elements, such as invading viruses. A central element of this immune system is an RNA-guided surveillance complex capable of targeting non-self DNA or RNA for degradation in a sequence- and site-specific manner analogous to RNA interference. Although the complexes display considerable diversity in their composition and architecture, many basic mechanisms underlying target recognition and cleavage are highly conserved. Using cryoelectron microscopy (cryo-EM), we show that the binding of target double-stranded DNA (dsDNA) to a type I-F CRISPR system yersinia (Csy) surveillance complex leads to large quaternary and tertiary structural changes in the complex that are likely necessary in the pathway leading to target dsDNA degradation by a trans-acting helicase-nuclease. Comparison of the structure of the surveillance complex before and after dsDNA binding, or in complex with three virally encoded anti-CRISPR suppressors that inhibit dsDNA binding, reveals mechanistic details underlying target recognition and inhibition.

PubMed: 28985564
DOI: 10.1016/j.cell.2017.09.006

Experimental method

X-RAY DIFFRACTION (2.265 Å)