5ZZ6

Redox-sensing transcriptional repressor Rex

Summary for 5ZZ6

• Entry DOI: 10.2210/pdb5zz6/pdb
• Descriptor: Redox-sensing transcriptional repressor Rex 1, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• Functional Keywords: redox sensing, transcription factor, gene regulation
• Biological source: Thermotoga maritima MSB8
• Total number of polymer chains: 4
• Total formula weight: 94364.05

Authors

Park, Y.W.,Jang, Y.Y.,Joo, H.K.,Lee, J.Y. (deposition date: 2018-05-30, release date: 2018-11-07, Last modification date: 2024-03-27)

Primary citation

Park, Y.W.,Jang, Y.Y.,Joo, H.K.,Lee, J.Y.
Structural Analysis of Redox-sensing Transcriptional Repressor Rex from Thermotoga maritima
Sci Rep, 8:13244-13244, 2018

PubMed Abstract: The cellular redox state of organisms continues to fluctuate during the metabolism. All organisms have various sensors that help detect and adapt to changes in the redox state. Nicotinamide adenine dinucleotides (NAD/NADH), which are involved in various cellular metabolic activities as cofactors, have been revealed as the key molecules sensing the intra-cellular redox state. The Rex family members are well conserved transcriptional repressors that regulate the expression of respiratory genes by sensing the redox state according to the intra-cellular NAD/NADH balance. Herein, we reported crystal structures of apo and NAD- and NADH-bound forms of Rex from Thermotoga maritima to analyse the structural basis of transcriptional regulation depending on either NAD or NADH binding. The different orientation of the reduced nicotinamide group to helix α9 caused the rearrangement of N-terminal DNA binding domain, thus resulting in closed form of Rex to dissociate from cognate DNA. The structural data of Rex from T. maritima also support the previous redox-sensing mechanism models of Rex homologues.

PubMed: 30185822
DOI: 10.1038/s41598-018-31676-z

Experimental method

X-RAY DIFFRACTION (2.2 Å)