5ZYF

Crystal structure of Streptococcus pyogenes type II-A Cas2

Summary for 5ZYF

• Entry DOI: 10.2210/pdb5zyf/pdb
• Descriptor: CRISPR-associated endoribonuclease Cas2, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: crispr, cas2, crispr-associated, streptococcus pyogenes, protein binding
• Biological source: Streptococcus pyogenes serotype M1
• Total number of polymer chains: 6
• Total formula weight: 65013.32

Authors

Ka, D.,Bae, E. (deposition date: 2018-05-24, release date: 2018-08-15, Last modification date: 2024-03-27)

Primary citation

Ka, D.,Jang, D.M.,Han, B.W.,Bae, E.
Molecular organization of the type II-A CRISPR adaptation module and its interaction with Cas9 via Csn2
Nucleic Acids Res., 46:9805-9815, 2018

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins provide microbial adaptive immunity against invading foreign nucleic acids. In type II-A CRISPR-Cas systems, the Cas1-Cas2 integrase complex and the subtype-specific Csn2 comprise the CRISPR adaptation module, which cooperates with the Cas9 nuclease effector for spacer selection. Here, we report the molecular organization of the Streptococcus pyogenes type II-A CRISPR adaptation module and its interaction with Cas9 via Csn2. We determined the crystal structure of S. pyogenes type II-A Cas2. Chromatographic and calorimetric analyses revealed the stoichiometry and topology of the type II-A adaptation module composed of Cas1, Cas2 and Csn2. We also demonstrated that Cas9 interacts with Csn2 in a direct and stoichiometric manner. Our results reveal a network of molecular interactions among type II-A Cas proteins and highlight the role of Csn2 in coordinating Cas components involved in the adaptation and interference stages of CRISPR-mediated immunity.

PubMed: 30102386
DOI: 10.1093/nar/gky702

Experimental method

X-RAY DIFFRACTION (1.76 Å)