5ZVN
Structure of [beta Glc-T9,K7]indolicidin, a glycosylated analogue of indolicidin
Summary for 5ZVN
| Entry DOI | 10.2210/pdb5zvn/pdb |
| Descriptor | glycosylated analogue of Indolicidin, beta-D-glucopyranose (2 entities in total) |
| Functional Keywords | antimicrobial indolicidin derivative, antimicrobial protein |
| Biological source | Bos taurus |
| Total number of polymer chains | 1 |
| Total formula weight | 2035.41 |
| Authors | Dwivedi, R.,Aggarwal, P.,Kaur, K.J.,Bhavesh, N.S. (deposition date: 2018-05-11, release date: 2019-06-19, Last modification date: 2020-07-29) |
| Primary citation | Dwivedi, R.,Aggarwal, P.,Bhavesh, N.S.,Kaur, K.J. Design of therapeutically improved analogue of the antimicrobial peptide, indolicidin, using a glycosylation strategy. Amino Acids, 51:1443-1460, 2019 Cited by PubMed Abstract: Indolicidin is a member of cathelicidin family which displays broad spectrum antimicrobial activity. Severe toxicity and aggregation propensity associated with indolicidin pose a huge limitation to its probable therapeutic application. We are reporting the use of glycosylation strategy to design an analogue of indolicidin and subsequently explore structural and functional effects of sugar on it. Our study led to the design of a potent antibacterial glycosylated peptide, [βGlc-T9,K7]indolicidin, which showed decreased toxicity against erythrocytes and macrophage cells and thus a higher therapeutic selectivity. The incorporation of sugar also increased the solubility of the peptide. The mode of bacterial killing, functional stability, LPS binding, and cytokine inhibitory potential of the peptide, however, seemed unaffected upon glycosylation. Absence of significant changes in structure upon glycosylation accounts for the possibly retained functions and mode of action of the peptide. Our report thus presents the designing of an indolicidin analogue with improved therapeutic potential by substituting aromatic amino acid with glycosylated amino acid as a promising strategy for the first time. PubMed: 31485742DOI: 10.1007/s00726-019-02779-2 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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