5ZTX

co-factor free Transaminase

Summary for 5ZTX

• Entry DOI: 10.2210/pdb5ztx/pdb
• Descriptor: transaminase, 1,2-ETHANEDIOL (3 entities in total)
• Functional Keywords: transaminase, transferase
• Biological source: Vibrio fluvialis
• Total number of polymer chains: 2
• Total formula weight: 103483.68

Authors

Park, H.H.,Shin, Y.C. (deposition date: 2018-05-05, release date: 2018-08-29, Last modification date: 2024-03-27)

Primary citation

Shin, Y.C.,Yun, H.,Park, H.H.
Structural dynamics of the transaminase active site revealed by the crystal structure of a co-factor free omega-transaminase from Vibrio fluvialis JS17
Sci Rep, 8:11454-11454, 2018

PubMed Abstract: Omega (ω)-transaminase catalyzes the transfer of an amino group from a non-α position amino acid, or an amine compound with no carboxylic group, to an amino acceptor, and has been studied intensively because of its high potential utility in industry and pharmatheutics. The ω-transaminase from Vibrio fluvialis JS17 (Vfat) is an amine:pyruvate transaminase capable of the stereo-selective transamination of arylic chiral amines. This enzyme exhibits extraordinary enantio-selectivity, and has a rapid reaction rate for chiral amine substrates. In this study, we report the crystal structure of the apo form of Vfat. The overall structure of Vfat was typical of other class III aminotransferase exhibiting an N-terminal helical domain, a small domain, and a large domain. Interestingly, the two subunits of apo Vfat in the asymmetric unit had different structures. A comparison of the overall structure to other transaminases, revealed that the structures of the N-terminal helical domain and the large domain can be affected by cofactor occupancy, but the structural rearrangement in these regions can occur independently.

PubMed: 30061559
DOI: 10.1038/s41598-018-29846-0

Experimental method

X-RAY DIFFRACTION (2 Å)