5ZRQ

Crystal structure of PET-degrading cutinase Cut190 S176A/S226P/R228S mutant in Zn(2+)-bound state

Summary for 5ZRQ

• Entry DOI: 10.2210/pdb5zrq/pdb
• Related: 5ZNO 5ZRR 5ZRS
• Descriptor: Alpha/beta hydrolase family protein, ZINC ION, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: polyesterase, alpha/beta-hydrolase fold, protein engineering, thermostability, hydrolase
• Biological source: Saccharomonospora viridis
• Total number of polymer chains: 1
• Total formula weight: 29949.13

Authors

Numoto, N.,Kamiya, N.,Bekker, G.J.,Yamagami, Y.,Inaba, S.,Ishii, K.,Uchiyama, S.,Kawai, F.,Ito, N.,Oda, M. (deposition date: 2018-04-25, release date: 2018-09-12, Last modification date: 2023-11-22)

• Primary citation: Numoto, N.,Kamiya, N.,Bekker, G.J.,Yamagami, Y.,Inaba, S.,Ishii, K.,Uchiyama, S.,Kawai, F.,Ito, N.,Oda, M.; Structural Dynamics of the PET-Degrading Cutinase-like Enzyme from Saccharomonospora viridis AHK190 in Substrate-Bound States Elucidates the Ca2+-Driven Catalytic Cycle.; Biochemistry, 57:5289-5300, 2018; PubMed: 30110540; DOI: 10.1021/acs.biochem.8b00624

Experimental method

X-RAY DIFFRACTION (1.12 Å)